Cell Image Identifier
Department
Molecular, Cellular, and Developmental Biology
Summary
A graduate student was doing a project that necessitated identification of individual cells and their nuclei in microscope fluorescence images and quantification of image characteristics within the cell/nuclei areas. With hundreds of cells per image, tens to hundreds of image layers per 3D exposure, and many exposures for the entire experiment, the process of manually tracing each cell and nucleus area in each image using the ImageJ analysis software was incredibly time consuming. Our group worked closely with the lab to develop a program that would automatically identify, outline, and number the cell and nucleus areas, as well as perform the desired analysis, saving the lab countless hours of work.